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dc.contributor.authorDoyle, E.
dc.contributor.authorAdams, Nigel
dc.date.accessioned2019-05-14T03:09:47Z
dc.date.available2019-05-14T03:09:47Z
dc.date.issued2018
dc.identifier.urihttps://hdl.handle.net/10652/4609
dc.description.abstractResearch which aims to identify the prey items found in the diet of seabirds by analysing stomach contents, faeces, and other remains strongly biased towards detecting organisms which are slow to digest, while soft bodied prey may be fully digested and remain undetected. DNA-based methods of prey item identification have been used in a number of studies to address this issue. The intention of the current research was to test the feasibility of applying DNA-based prey item identification to analysing the diet of the Buller’s shearwater (Puffinis bulleri). DNA was extracted from 17 samples of regurgitate and faecal material collected from Buller’s shearwaters, received from the Northern New Zealand Seabird Trust. Following DNA extraction, PCR amplification is used to selectively amplify short sections of the 16S gene which can be used for identification. PCR products were run on an agarose gel, as a success/fail test for amplification. Only one of the DNA samples tested could be amplified. While this result suggests that DNA-based analysis may not be suitable for research on the diet of the Buller’s shearwater, the outcome may be due to the small scale of the test.en_NZ
dc.language.isoenen_NZ
dc.publisherUnitec Institute of Technology. Applied Molecular Solutionsen_NZ
dc.subjectNew Zealand birdsen_NZ
dc.subjectBuller’s shearwaters (Puffinis bulleri)en_NZ
dc.subjectPuffinis bullerien_NZ
dc.subjectfaecal samplesen_NZ
dc.subjectDNA barcodingen_NZ
dc.subjectDNA extractionen_NZ
dc.titleDNA extraction and amplification of regurgitate and faecal samples from Buller’s Shearwater (Puffinis bulleri). Report prepared for Northern New Zealand Seabird Trusten_NZ
dc.typeOtheren_NZ
dc.date.updated2019-04-16T14:30:13Z
dc.subject.marsden060411 Population, Ecological and Evolutionary Geneticsen_NZ
dc.identifier.bibliographicCitationDoyle, E., & Adams, N. (2018). DNA extraction and amplification of regurgitate and faecal samples from Buller’s Shearwater (Puffinis bulleri). Report prepared for Northern New Zealand Seabird Trust . 8 p. Auckland:en_NZ
unitec.peerreviewedyesen_NZ
unitec.identifier.roms63204en_NZ
unitec.publication.placeMount Albert, Auckland, New Zealanden_NZ


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