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dc.contributor.authorDoyle, E.
dc.contributor.authorAdams, Nigel
dc.date.accessioned2019-05-08T00:48:40Z
dc.date.available2019-05-08T00:48:40Z
dc.date.issued2018
dc.identifier.urihttps://hdl.handle.net/10652/4607
dc.description.abstractThe intention of the current research was to test the feasibility of applying DNA-based prey item identification to analysing the diet of three New Zealand sea bird species, using ethanol preserved faecal samples. DNA was extracted from 20 faecal samples collected from Buller’s shearwaters (Puffinis bulleri), Fluttering Shearwaters (Puffinus gavial), and Fairy prions (Pachyptila turtur), received from the Northern New Zealand Seabird Trust. PCR amplification was then used to selectively amplify short sections of the 16S gene which can be used for identification. PCR products were run on an agarose gel, as a success/fail test for amplification. DNA was successfully amplified in only three of the twenty samples despite suitably high nucleotide concentrations in the DNA extracts, indicating that the primers used may not be suitable for this research.en_NZ
dc.language.isoenen_NZ
dc.publisherUnitec Institute of Technology. Applied Molecular Solutionsen_NZ
dc.subjectNew Zealand birdsen_NZ
dc.subjectBuller’s shearwaters (Puffinis bulleri)en_NZ
dc.subjectPuffinis bullerien_NZ
dc.subjectFluttering shearwaters (Puffinus gavial)en_NZ
dc.subjectPuffinis gavialen_NZ
dc.subjectFairy prions (Pachyptila turtur)en_NZ
dc.subjectPachyptila turturen_NZ
dc.subjectfaecal samplesen_NZ
dc.subjectDNA barcodingen_NZ
dc.subjectDNA extractionen_NZ
dc.titleDNA extraction and amplification of faecal samples from Buller’s Shearwater (Puffinis bulleri), Fairy Prion (Pachyptila turtur), & Fluttering Shearwater (Puffinus gavia). Report prepared for North New Zealand Seabird Trust. 7 p.en_NZ
dc.typeOtheren_NZ
dc.date.updated2019-04-16T14:30:13Z
dc.subject.marsden060411 Population, Ecological and Evolutionary Geneticsen_NZ
dc.identifier.bibliographicCitationDoyle, E., & Adams, N. (2018). DNA extraction and amplification of faecal samples from Buller’s Shearwater (Puffinis bulleri), Fairy Prion (Pachyptila turtur), & Fluttering Shearwater (Puffinus gavia). Report prepared for North New Zealand Seabird Trust. 7 p. Auckland:en_NZ
unitec.peerreviewedyesen_NZ
unitec.identifier.roms63205en_NZ
unitec.publication.placeMount Albert, Auckland, New Zealanden_NZ


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