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dc.contributor.authorDoyle, E.
dc.contributor.authorAdams, Nigel
dc.date.accessioned2019-05-07T01:12:49Z
dc.date.available2019-05-07T01:12:49Z
dc.date.issued2019
dc.identifier.urihttps://hdl.handle.net/10652/4606
dc.description.abstractThis report describes the success of DNA extraction and amplification of fresh faecal samples collected from White Fronted Tern Sterna striata in the field. Sample handling involved immediate placement in ethanol field followed by freezing at -20oC a few hours later on the day of collection.. DNA was extracted from 10 faecal samples collected from birds (Sternula nereis). PCR using primers targeting Chordata was then used to selectively amplify short sections of the 16S gene which can be used for prey identification . PCR products were run on an agarose gel, as a success/fail test for amplification. DNA was successfully amplified in four of the ten samples. Attempts to amplify DNA using primers targeting Malacostraca and a second pair of PCR primers, targeting a 180-270bp section of the 16S gene of multiple taxa, were unsuccessful.en_NZ
dc.language.isoenen_NZ
dc.publisherUnitec Institute of Technology. Applied Molecular Solutionsen_NZ
dc.subjectNew Zealand birdsen_NZ
dc.subjectWhite Fronted Tern (Sternula nereis)en_NZ
dc.subjectSternula nereisen_NZ
dc.subjectfaecal samplesen_NZ
dc.subjectDNA barcodingen_NZ
dc.subjectDNA extractionen_NZ
dc.titleDNA extraction and amplification of faecal samples from the White Fronted Terns (Sternula nereis). Report prepared for North New Zealand Seabird Trust. 6 p.en_NZ
dc.typeOtheren_NZ
dc.date.updated2019-04-16T14:30:14Z
dc.subject.marsden060411 Population, Ecological and Evolutionary Genetics
dc.identifier.bibliographicCitationDoyle, E., & Adams, N. (2019). DNA extraction and amplification of faecal samples from the White Fronted Terns (Sternula nereis). Report prepared for North New Zealand Seabird Trust. 6 p. Auckland.en_NZ
unitec.peerreviewedyesen_NZ
unitec.identifier.roms63206en_NZ
unitec.publication.placeMount Albert, Auckland, New Zealanden_NZ


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