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    Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae)

    Bulgarella, M.; Biggs, Patrick J.; de Lange, Peter; Shepherd, L.D.

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    Bulgarella_et_al._2017_Arthropodium_Microsatellites_APS.pdf (525.6Kb)
    Date
    2017-08-25
    Citation:
    Bulgarella, M., Biggs, P., de Lange, P.J., & Shepherd, L. (2017). Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae). Applications of Plant Sciences, 5, pp.1700041. doi:doi.org/10.3732/apps.1700041
    Permanent link to Research Bank record:
    https://hdl.handle.net/10652/4119
    Abstract
    • PREMISE OF THE STUDY: Microsatellite markers were developed for Arthropodium cirratum (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other Arthropodium species. • METHODS AND RESULTS: Sixteen microsatellite markers were developed from a genomic library and tested in three populations of A. cirratum. The loci exhibited one to five alleles per locus, with private alleles present in each of the populations. Cross-amplification tests in the two other New Zealand Arthropodium species revealed that many of the loci amplify and demonstrate polymorphism in A. bifurcatum. • CONCLUSIONS: These markers will be useful for determining genetic structure in A. cirratum and for determining the origins of translocated populations of this species.
    Keywords:
    Arthropodium, Asparagaceae, nuclear microsatellites, rengarenga, translocation
    ANZSRC Field of Research:
    060310 Plant Systematics and Taxonomy
    Copyright Notice:
    Copyright © 2018 BioOne All rights reserved.
    Available Online at:
    http://www.bioone.org/doi/full/10.3732/apps.1700041
    Rights:
    This digital work is protected by copyright. It may be consulted by you, provided you comply with the provisions of the Act and the following conditions of use: Any use you make of these documents or images must be for research or private study purposes only, and you may not make them available to any other person. You will recognise the author's and publishers rights and give due acknowledgement where appropriate.
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