Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae)
Date
2017-08-25Citation:
Bulgarella, M., Biggs, P., de Lange, P.J., & Shepherd, L. (2017). Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae). Applications of Plant Sciences, 5, pp.1700041. doi:doi.org/10.3732/apps.1700041Permanent link to Research Bank record:
https://hdl.handle.net/10652/4119Abstract
• PREMISE OF THE STUDY:
Microsatellite markers were developed for Arthropodium cirratum (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other Arthropodium species.
• METHODS AND RESULTS:
Sixteen microsatellite markers were developed from a genomic library and tested in three populations of A. cirratum. The loci exhibited one to five alleles per locus, with private alleles present in each of the populations. Cross-amplification tests in the two other New Zealand Arthropodium species revealed that many of the loci amplify and demonstrate polymorphism in A. bifurcatum.
• CONCLUSIONS:
These markers will be useful for determining genetic structure in A. cirratum and for determining the origins of translocated populations of this species.